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Fabrication of Multielectrode Arrays for Neurobiology Applications

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Book cover Cell-Based Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1771))

Abstract

Substrate-integrated multielectrode arrays (MEAs) enable multisite, long-term, and label-free sensing and actuation of neuronal electrical signals in reduced cell culture models for network electrophysiology. Conventional, thin-film fabricated passive MEAs typically provide a few tens of electrode sites. New generations of active CMOS-based high-resolution arrays provide the capabilities of simultaneous recordings from thousands of neurons over fields of view of several square millimeters, yet allowing extracellular electrical imaging to be achieved down to the subcellular scale. In turn, such advancement in chip-based electrical readouts can significantly complement recently developed biotechnological and bimolecular techniques for neurobiology applications. Here, we describe (1) a simple method to fabricate passive MEAs and (2) protocols for preparing and growing primary rat hippocampal neuronal cultures and human iPS-derived neurons on MEAs. The aim is to provide reliable protocols for initiating the reader to this technology and for stimulating their further development and experimental use in neurobiology.

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Authors and Affiliations

  1. Fondazione Istituto Italiano di Tecnologia (IIT), Neuroscience and Brain Technologies, Genoa, Italy

    Mario Malerba, Hayder Amin, Gian N. Angotzi, Alessandro Maccione & Luca Berdondini

Authors
  1. Mario Malerba
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  2. Hayder Amin
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  3. Gian N. Angotzi
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  4. Alessandro Maccione
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  5. Luca Berdondini
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Corresponding author

Correspondence to Luca Berdondini .

Editor information

Editors and Affiliations

  1. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Peter Ertl

  2. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Mario Rothbauer

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Malerba, M., Amin, H., Angotzi, G.N., Maccione, A., Berdondini, L. (2018). Fabrication of Multielectrode Arrays for Neurobiology Applications. In: Ertl, P., Rothbauer, M. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 1771. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7792-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7792-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7791-8

  • Online ISBN: 978-1-4939-7792-5

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