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High-Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluripotent Stem Cells

  • Shyam Sushama Jose
  • Kamila Bendickova
  • Jan Fric
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

The successful development and characterization of human induced pluripotent stem cells (iPSCs) provides a powerful tool to study the molecular mechanisms that control cell fate decisions and differentiation toward distinct lineages. Here we focus on the ability of donors derived iPSCs to differentiate toward hematopoietic progenitor cells and on the analysis of their telomere length. The ability to screen telomere length in individual donors is important for defining cellular senescence, which correlates with their differentiation potential toward hematopoietic lineages. We have modified iPSC culture protocol and telomere length analysis to suit for high throughput screening of telomere length in large number of individual donors. This approach can be used to demonstrate the heterogeneity or changes of telomere length and its shortening as an exclusion criterion for selection of suitable donors for future stem cell therapies.

Key words

Induced pluripotent stem cells Hematopoiesis Hematopoietic stem cells Cellular senescence Telomere length 

Notes

Acknowledgment

Supported by European Social Fund and European Regional Development Fund—Project MAGNET (No. CZ.02.1.01/0.0/0.0/15_003/0000492). SSJ is a recipient of Masaryk University Faculty of Medicine Stipend for doctoral studies.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shyam Sushama Jose
    • 1
  • Kamila Bendickova
    • 1
  • Jan Fric
    • 1
  1. 1.Cellular and Molecular Immunoregulation Group (CMI), Center for Translational Medicine (CTM)International Clinical Research Center (ICRC), St. Anne’s University Hospital BrnoBrnoCzech Republic

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