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Photosynthesis pp 197-211 | Cite as

Liquid-Phase Measurements of Photosynthetic Oxygen Evolution

  • Dmitriy Shevela
  • Wolfgang P. Schröder
  • Johannes Messinger
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1770)

Abstract

This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.

Key words

Photosynthetic water oxidation O2 evolution Photosystem II Clark-type electrode Membrane inlet mass spectrometry 

Notes

Acknowledgments

We thank Dr. Mun Hon Cheah for careful reading of the manuscript and valuable suggestions. The Swedish Energy Agency (Energimyndigheten), Swedish Science Foundation (Vetenskapsrådet), and the K & A Wallenberg Foundation are acknowledged for financial support.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryUmeå UniversityUmeåSweden
  2. 2.Department of Chemistry - Ångström LaboratoryUppsala UniversityUppsalaSweden

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