Orchid Seed Germination and Micropropagation I: Background Information and Related Protocols

  • Edward C. YeungEmail author
  • Jungmin Park
  • Indra S. Harry
Part of the Springer Protocols Handbooks book series (SPH)


Seed germination and micropropagation of orchids are key approaches to orchid conservation and commercial production. The general concepts and methodologies to orchid asymbiotic and symbiotic seed germination and regeneration are clearly established in the literature. This chapter discusses the general techniques and common culture media used in in vitro studies. Also, frequently used additives and related protocols on sterilization techniques and viability staining of seeds are documented.

Key words

Asymbiotic seed germination Symbiotic seed germination Culture media Media additives Sterilization protocols Viability staining 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Edward C. Yeung
    • 1
    Email author
  • Jungmin Park
    • 1
  • Indra S. Harry
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

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