In Vitro Flowering and Breeding of Erycina pusilla

  • Yi-Tien Chiu
  • Chen ChangEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Erycina pusilla is a miniature orchid species native to tropical America. The unique characteristics, e.g., a short juvenile stage and its miniature size, make E. pusilla a perfect material for producing the ornamental flask product and for the study of flowering physiology and in vitro breeding. In this chapter, we demonstrate the culture steps of in vitro flowering of E. pusilla. Using method detailed, it is possible to produce blooming E. pusilla plants within 1 year from in vitro seed germination and producing ornamental flasks for commercial sale. This culture protocol can also be used for in vitro breeding to select additional desirable traits for ornamental flask production.

Key words

Erycina pusilla flowering Miniature orchids Breeding Pollination Subcultured In vitro ornamental orchid product 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of HorticultureNational Chung Hsing UniversityTaichung CityRepublic of China

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