Intravital Imaging of T Cells Within the Spinal Cord
Intravital imaging is a powerful tool for analyzing cellular functions in living animals. In particular, after the two-photon microscopy technique was introduced, a number of studies have visualized important processes. Here, we describe the methods for performing intravital imaging of the central nervous system. This method can be used for imaging not only lymphocytes but also blood vessels for ischemia studies, as well as glia cell activities.
Key wordsTwo-photon microscopy Intravital imaging Autoimmunity Central nervous system T cells
This work was supported by DFG (Transregio 128, Heisenberg fellowship and individual grant KA2951/2-1), the Novartis Foundation for Therapeutic Research, Max-Planck Society, and LMU Munich.