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Root Gravitropism: Quantification, Challenges, and Solutions

  • Lukas Muller
  • Malcolm J. Bennett
  • Andy French
  • Darren M. Wells
  • Ranjan Swarup
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1761)

Abstract

Better understanding of root traits such as root angle and root gravitropism will be crucial for development of crops with improved resource use efficiency. This chapter describes a high-throughput, automated image analysis method to trace Arabidopsis (Arabidopsis thaliana) seedling roots grown on agar plates. The method combines a “particle-filtering algorithm with a graph-based method” to trace the center line of a root and can be adopted for the analysis of several root parameters such as length, curvature, and stimulus from original root traces.

Key words

Root gravitropism RootTrace Image analysis Arabidopsis 

Notes

Acknowledgment

We acknowledge support from the Biological and Biotechnological Science Research Council (BBSRC), European Research Council (ERC), Royal Society, and Newton International and Advanced Fellowship awards.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Lukas Muller
    • 1
  • Malcolm J. Bennett
    • 1
  • Andy French
    • 1
  • Darren M. Wells
    • 1
  • Ranjan Swarup
    • 1
  1. 1.Centre for Plant Integrative Biology, School of BiosciencesUniversity of NottinghamLoughboroughUK

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