DNA Genome Sequencing in Esophageal Adenocarcinoma

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1756)

Abstract

Next-generation sequencing refers to the high-throughput DNA sequencing technologies, which are capable of sequencing large numbers of different DNA sequences in a single/parallel reaction. It is a powerful tool to identify inherited and acquired genetic alterations associated with the development of esophageal adenocarcinoma. Whole-genome sequencing is the most comprehensive but expensive, whereas whole-exome sequencing is cost-effective but it only works for the known genes. Thus, second-generation sequencing methods can provide a complete picture of the esophageal adenocarcinoma genome by detecting and discovering different type of alterations in the cancer. This would help in diagnostics and will further help in developing personalized medicine in esophageal adenocarcinoma.

Key words

Esophageal adenocarcinoma Next-generation sequencing Whole-genome sequencing Whole-exome sequencing DNA 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia
  2. 2.School of Biomedical Sciences, Faculty of MedicineUniversity of QueenslandBrisbaneAustralia

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