Embryonary Mouse Cardiac Fibroblast Isolation

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1752)

Abstract

Mouse cardiac fibroblasts have been widely used as an in vitro model for studying fundamental biological processes and mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Cardiac FBs are relatively easy to culture in a dish and can be manipulated using molecular and pharmacological tools. Because FBs rapidly decrease cell cycle division and proliferative rate after birth, they are prone to phenotypic changes and senescence in cell culture soon after a few passages. Therefore, primary cultures of differentiated fibroblasts from embryos are more desirable. Below we will describe a method that provides good cell yield and viability of E16 CD-1 mouse embryonic cardiac fibroblasts in primary cultures.

Key words

Cardiac fibroblasts Heart Enzymatic digestion Embryonic mice Isolation Cell culture 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medicine, School of MedicineUniversity of CaliforniaSan DiegoUSA
  2. 2.Escuela de MedicinaUniversidad PanamericanaMéxico, Cuidad de MéxicoMexico

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