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Analysis of RNA Editing Sites from RNA-Seq Data Using GIREMI

  • Qing Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1751)

Abstract

RNA editing is a posttranscriptional modification process that alters the sequence of RNA molecules. RNA editing is related to many human diseases. However, the identification of RNA editing sites typically requires matched genomic sequence or multiple related expression data sets. Here we describe the GIREMI tool (genome-independent identification of RNA editing by mutual information; https://github.com/zhqingit/giremi) that is designed to accurately and sensitively predict adenosine-to-inosine editing from a single RNA-Seq data set.

Key words

RNA editing RNA-Seq Posttranscriptional modification 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Qing Zhang
    • 1
  1. 1.Integrative Biology and PhysiologyThe University of California, Los Angeles (UCLA)Los AngelesUSA

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