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MicroRNA Profiling of Alzheimer’s Disease Cerebrospinal Fluid

  • Johannes Denk
  • Holger Jahn
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1750)

Abstract

MicroRNAs (miRNAs) are a class of small, highly conserved, and noncoding RNAs that modulate gene expression by regulating the activity and stability of target mRNAs. MiRNAs play significant roles by controlling fundamental cellular processes and its deregulation is associated with various diseases. Ubiquitous expression and its release into circulation make them interesting biomarkers, which can be measured by different platforms. In this book chapter, we provide a specific protocol that describes the detection of circulating miRNAs in CSF by using RT-qPCR.

Key words

RT-qPCR CSF MicroRNA MIQE Biomarker SYBR-green CNS disorders 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Psychiatry and PsychotherapyUniversity Medical Center Hamburg-EppendorfHamburgGermany

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