MicroRNA Profiling of Alzheimer’s Disease Cerebrospinal Fluid
MicroRNAs (miRNAs) are a class of small, highly conserved, and noncoding RNAs that modulate gene expression by regulating the activity and stability of target mRNAs. MiRNAs play significant roles by controlling fundamental cellular processes and its deregulation is associated with various diseases. Ubiquitous expression and its release into circulation make them interesting biomarkers, which can be measured by different platforms. In this book chapter, we provide a specific protocol that describes the detection of circulating miRNAs in CSF by using RT-qPCR.
Key wordsRT-qPCR CSF MicroRNA MIQE Biomarker SYBR-green CNS disorders
- 3.Balusu S, Van Wonterghem E, De Rycke R et al (2016) Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles. EMBO Mol Med 8(10):1162–1183. https://doi.org/10.15252/emmm.201606271 CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64(15):5245–5250. https://doi.org/10.1158/0008-5472.CAN-04-0496 CrossRefPubMedGoogle Scholar