A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
An emerging body of evidences based on in vitro studies indicate that mitophagy (selective autophagic clearance of damaged mitochondria) is a prosurvival mechanism, specifically under exposure to various stressors. Sertoli cells (SCs) play essential roles in maintenance of spermatogenesis via paracrine interactions with germ cells and other somatic cells in the testis; however, studies investigating mitophagy in SCs are still very few. In this chapter, we give a brief review of mechanisms and detection methods of mitophagy in SCs based on our recent publications on animal models of ethanol toxicity and current literature. In addition, we provide a method for induction and ultrastructural identification of mitophagy in SCs of adult Wistar rats using a single intraperitoneal injection (5 g/kg) of ethanol. Proper understanding of mitophagy features and mechanisms in SCs may have therapeutic implications for infertility associated with alcoholism and other diseases characterized by mitochondrial dysfunction.
KeywordsAutophagy Ethanol Mitophagy Sertoli cell TEM
This manuscript is partially supported by Grant-in-Aid for Scientific Research (Kakenhi) (No., 16K11073) from The Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Prof. Yoshinori Otsuki for critical advice and support of this study.
- 18.Betsuyaku T, Eid N, Ito Y et al (2017) Ethanol enhances thymocyte apoptosis and autophagy in macrophages of rat thymi. Histol Histopathol 32:963–975Google Scholar