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Sertoli Cells pp 279-286 | Cite as

A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells

  • Anna Maggio
  • Raquel L. Bernardino
  • Patrizia Gena
  • Marco G. Alves
  • Pedro F. Oliveira
  • Giuseppe CalamitaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1748)

Abstract

Movement of water into and out of the cell is fundamental to life. In the male reproductive tract, at the level of the seminiferous epithelium, Sertoli cells are pivotal in mediating the movement of water into the luminal compartment, a process providing a means of transport of spermatozoa into the epididymal ducts. Here, we describe a stopped-flow light-scattering methodology to easily assess the Sertoli cell osmotic water permeability. The devised methodology is well suited for studying how the Sertoli cell’s permeability changes following exogenous stimuli.

Keywords

Aquaporin channels Male fertility Membrane water permeability Sertoli cells Spermatogenesis 

Notes

Acknowledgment

This work was supported by the “Fundação para a Ciência e a Tecnologia” (FCT) (PTDC/BBB-BQB/1368/2014), UMIB (PEst-OE/SAU/UI0215/2014). R.L. Bernardino was also financed by (SFRH/BD/103105/2014).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Anna Maggio
    • 1
    • 2
  • Raquel L. Bernardino
    • 2
  • Patrizia Gena
    • 1
  • Marco G. Alves
    • 2
  • Pedro F. Oliveira
    • 3
    • 4
    • 5
    • 1
  • Giuseppe Calamita
    • 1
    Email author
  1. 1.Department of Biosciences, Biotechnologies and BiopharmaceuticsUniversity of Bari “Aldo Moro”BariItaly
  2. 2.Unit for Multidisciplinary Research in Biomedicine (UMIB), Laboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS)University of PortoPortoPortugal
  3. 3.Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS)University of PortoPortoPortugal
  4. 4.Department of Genetics, Faculty of MedicineUniversity of PortoPortoPortugal
  5. 5.i3S - Instituto de Investigação e Inovação em SaúdeUniversity of PortoPortoPortugal

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