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The EPR Method for Detecting Nitric Oxide in Plant Senescence

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Plant Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1744))

Abstract

Nitric oxide (NO) is gaining increasing attention as a central molecule with diverse signaling functions. It has been shown that NO acts as a negative regulator of leaf senescence. In this chapter, we describe a highly selective method, electron paramagnetic resonance ([EPR], also known as electron spin resonance [ESR]), for NO determination in leaf senescence. An iron complex of ferrous and mononitrosyl dithiocarbamate (Fe2+(DETC)2) is used as a chelating agent for NO. Using ethyl acetate as extracting solvent, the NOFe2+(DETC)2 complex is extracted and determined by EPR spectrometer.

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Authors and Affiliations

  1. The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

    Aizhen Sun

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  1. Aizhen Sun
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Correspondence to Aizhen Sun .

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Editors and Affiliations

  1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, China

    Yongfeng Guo

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Sun, A. (2018). The EPR Method for Detecting Nitric Oxide in Plant Senescence. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7672-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7670-6

  • Online ISBN: 978-1-4939-7672-0

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