Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants

  • Qingyu Wu
  • David JacksonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1743)


Programmed cell death (PCD) is an essential component of development, biotic and abiotic responses. Hypersensitive response (HR)-associated cell death activated under pathogen attack is one of the most dramatic manifestations of PCD in plants. Signal transduction through mitogen-activated protein kinase (MAPK) cascades, a very conserved signaling pathway across eukaryotes, is a core mediator for HR-associated PCD. Therefore, monitoring MAPK activation enables the mechanisms underlying HR-associated PCD to be elucidated. Here, we describe the use of a phosphorylation-specific MAPK3/6 antibody to monitor the activation of MAPK3/6 during HR-associated PCD. The technique may be adapted for use in other types of PCD.


Programmed cell death MAPK Hypersensitive response Phosphorylation 



Q.W. and D.J. are supported by the AFRI grant no. 2013-02198 of the USDA National Institute of Food and Agriculture.


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© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA

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