Oxidation of membrane lipids by reactive oxygen species primarily generates lipid peroxides, from which various carbonyls, i.e., aldehydes and ketones, are formed. Among them, those with a carbonyl-conjugated C-C double bond have significant biological functions and are designated as reactive carbonyl species (RCS). A dozen kinds of RCS occurring in plant cells have a broad spectrum of reactivity and biological effects, depending on the structure. Several RCS have been recently found to activate caspase-like proteases in plants, thereby initiating PCD. Comprehensive and quantitative RCS analysis method using conventional HPLC is illustrated.
HPLC 2,4-Dinitrophenylhydrazine Reactive carbonyl species Reactive electrophile species
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
Mano J (2012) Reactive carbonyl species: their production from lipid peroxides, action in environmental stress, and the detoxification mechanism. Plant Physiol Biochem 59:90–97CrossRefPubMedGoogle Scholar
Farmer EE, Mueller MJ (2013) ROS-mediated lipid peroxidation and RES-activated signaling. Annu Rev Plant Biol 64:429–450CrossRefPubMedGoogle Scholar
Esterbauer H, Schauer R, Zollner JH (1991) Chemistry and biochemistry of 4-hydroxynonenal, malondialdehyde and related aldehydes. Free Radic Biol Med 11:81–128CrossRefPubMedGoogle Scholar
Reynolds T (1977) Comparative effects of aliphatic compounds on inhibition of lettuce fruit germination. Ann Bot 41:637–648CrossRefGoogle Scholar
Biswas MS, Mano J (2015) Lipid peroxide-derived short-chain carbonyls mediate hydrogen peroxide-induced and salt-induced programmed cell death in plants. Plant Physiol 168:885–898CrossRefPubMedPubMedCentralGoogle Scholar
Srivastava S, Brychkva G, Yarmolinsky D, Soltabyeva A, Samani T, Sagi M (2017) Aldehyde oxidase 4 plays a critical role in delaying silique senescence by catalyzing aldehyde detoxification. Plant Physiol 173:1977–1997CrossRefPubMedPubMedCentralGoogle Scholar
Matsui K, Sugimoto K, Kakyumyan P et al (2009) Volatile oxylipins and related compounds formed under stress in plants. In: Armstrong D (ed) Methods in molecular biology ‘Lipidomics’. Humana Press, Totowa, pp 17–28Google Scholar
Mano J, Khorobrykh S, Matsui K et al (2014) Acrolein is formed from trienoic fatty acids in chloroplasts: a targeted metabolomics approach. Plant Biotechnol 31:535–544CrossRefGoogle Scholar