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Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress

  • Jun’ichi ManoEmail author
  • Md. Sanaullah Biswas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1743)

Abstract

Oxidation of membrane lipids by reactive oxygen species primarily generates lipid peroxides, from which various carbonyls, i.e., aldehydes and ketones, are formed. Among them, those with a carbonyl-conjugated C-C double bond have significant biological functions and are designated as reactive carbonyl species (RCS). A dozen kinds of RCS occurring in plant cells have a broad spectrum of reactivity and biological effects, depending on the structure. Several RCS have been recently found to activate caspase-like proteases in plants, thereby initiating PCD. Comprehensive and quantitative RCS analysis method using conventional HPLC is illustrated.

Keywords

HPLC 2,4-Dinitrophenylhydrazine Reactive carbonyl species Reactive electrophile species 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Science Research Center, Organization for Research InitiativesYamaguchi UniversityYamaguchiJapan
  2. 2.Department of HorticultureBangabandhu Agricultural UniversityGazipurBangladesh

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