Isolation of Extracellular RNA from Bile
The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.
KeywordsExtracellular Vesicles Bile Isolation Extracellular RNA Exosomes
This work was supported by the Office of the Director, National Institutes of Health (USA) through award UH3 TR000884. We acknowledge the expert assistance of Caitlyn Foerst and thank the members of our laboratories for their contributions.