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Isolation of Extracellular RNA from Serum/Plasma

  • Justyna Filant
  • Parham Nejad
  • Anu Paul
  • Bridget Simonson
  • Srimeenakshi Srinivasan
  • Xuan Zhang
  • Leonora Balaj
  • Saumya Das
  • Roopali Gandhi
  • Louise C. LaurentEmail author
  • Anil K. Sood
Part of the Methods in Molecular Biology book series (MIMB, volume 1740)

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

Keywords

Cell biology Extracellular RNA Extracellular Vesicle Nucleic acids Molecular Biology Isolation Purification and separation 

Notes

Acknowledgements

J.F. and A.S. were supported by NIH grant UH3 TR000943. P.N., A.P., and R.G. were supported by UH2-UH3 TR000890. B.S. and S.D. were supported by UH3TR000901. S.S. and L.L. were supported by U01 HL126494 and UH3 TR000906. X.Z. and L.B. were supported by U19 CA179463. Christina Bennett helped in proofreading and formatting the article.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Justyna Filant
    • 1
  • Parham Nejad
    • 2
  • Anu Paul
    • 2
  • Bridget Simonson
    • 3
  • Srimeenakshi Srinivasan
    • 4
  • Xuan Zhang
    • 5
  • Leonora Balaj
    • 5
    • 6
  • Saumya Das
    • 3
  • Roopali Gandhi
    • 2
  • Louise C. Laurent
    • 4
    Email author
  • Anil K. Sood
    • 1
    • 7
    • 8
  1. 1.Department of Gynecologic Oncology and Reproductive MedicineThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  2. 2.Department of Neurology, Center for Neurologic Diseases, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  3. 3.Cardiovascular Research CenterMassachusetts General Hospital, Harvard Medical SchoolBostonUSA
  4. 4.Department of Reproductive MedicineUniversity of California, San DiegoLa JollaUSA
  5. 5.Department of NeurologyMassachusetts General Hospital, Harvard Medical SchoolBostonUSA
  6. 6.Program in NeuroscienceMassachusetts General Hospital, Harvard Medical SchoolBostonUSA
  7. 7.Center for RNA Interference and Non-Coding RNAsMDACCHoustonUSA
  8. 8.Department of Cancer BiologyMDACCHoustonUSA

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