Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
As endogenous biological nanoparticles capable of uptake by cells, extracellular vesicles (EVs) have the capacity to deliver their RNA cargo to recipient cells. The use of EVs as a drug delivery system remains in its infancy, and there are several barriers to the use of EV for this purpose. Amongst these is the need to ensure that adequate amounts of EV are available. The use of milk-derived EV provides a scalable approach and loading of these EVs with RNA is possible with the use of chemical transfection reagents. This method describes the use of milk-derived EV for delivery of small interfering RNA. These EVs were shown to be taken up by hepatocellular carcinoma cells in vitro, with a reduction in the expression of target gene.
KeywordsDrug delivery Extracellular vesicles Milk siRNA Drug loading method Lipofection Ultracentrifugation
This work was supported by the National Institutes of Health (USA) Office of the Director through grant UH3 TR000884. We acknowledge the expert assistance of Caitlyn Foerst and thank the members of our laboratories for their contributions.