Advertisement

Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1736)

Abstract

The study of the dynamics that occur during the course of a bacterial infection has been attempted using several methods. Here we discuss the construction of a set of antibiotic-resistant, otherwise-isogenic Staphylococcus aureus strains that can be used to observe the progress of systemic disease in a mouse model at various time-points postinfection. The strains can likewise be used to study the progression of infection in other animal infection models, such as the zebrafish embryo. Furthermore, the use of antibiotic resistance tags provides a convenient system with which to investigate the effect of antimicrobial chemotherapy during disease.

Key words

Infection Dynamics Mutant Isogenic Animal model 

References

  1. 1.
    Law V, Knox C, Djoumbou Y et al (2013) DrugBank 4.0: shedding new light on drug metabolism. Nucleic Acids Res 42(Database issue):D1091–D1097.  https://doi.org/10.1093/nar/gkt1068 PubMedPubMedCentralGoogle Scholar
  2. 2.
    Horst SA, Hoerr V, Beineke A et al (2012) A novel mouse model of Staphylococcus Aureus chronic osteomyelitis that closely mimics the human infection: an integrated view of disease pathogenesis. Am J Pathol 181:1206–1214CrossRefPubMedGoogle Scholar
  3. 3.
    Hume EB, Cole N, Khan S et al (2005) A Staphylococcus aureus mouse keratitis topical infection model: cytokine balance in different strains of mice. Immunol Cell Biol 83:294–300CrossRefPubMedGoogle Scholar
  4. 4.
    McVicker G, Prajsnar TK, Williams A et al (2014) Clonal expansion during Staphylococcus aureus infection dynamics reveals the effect of antibiotic intervention. PLoS Pathog 10:e1003959CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Grant AJ, Restif O, McKinley TJ et al (2008) Modelling within-host spatiotemporal dynamics of invasive bacterial disease. PLoS Biol 6:e74CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Prajsnar TK, Hamilton R, Garcia-Lara J et al (2012) A privileged intraphagocyte niche is responsible for disseminated infection of Staphylococcus aureus in a zebrafish model. Cell Microbiol 14:1600–1619CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Krebs InstituteUniversity of SheffieldSheffieldUK
  2. 2.Department of Molecular Biology and BiotechnologyUniversity of SheffieldSheffieldUK
  3. 3.Bateson CentreUniversity of SheffieldSheffieldUK

Personalised recommendations