Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics

Part of the Methods in Molecular Biology book series (MIMB, volume 1736)


The study of the dynamics that occur during the course of a bacterial infection has been attempted using several methods. Here we discuss the construction of a set of antibiotic-resistant, otherwise-isogenic Staphylococcus aureus strains that can be used to observe the progress of systemic disease in a mouse model at various time-points postinfection. The strains can likewise be used to study the progression of infection in other animal infection models, such as the zebrafish embryo. Furthermore, the use of antibiotic resistance tags provides a convenient system with which to investigate the effect of antimicrobial chemotherapy during disease.

Key words

Infection Dynamics Mutant Isogenic Animal model 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Krebs InstituteUniversity of SheffieldSheffieldUK
  2. 2.Department of Molecular Biology and BiotechnologyUniversity of SheffieldSheffieldUK
  3. 3.Bateson CentreUniversity of SheffieldSheffieldUK

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