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Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1736)

Abstract

Investigating bacterial dynamics within the infected host has proved very useful for understanding mechanisms of pathogenesis. Here we present the protocols we use to study bacterial dynamics within infected embryonic zebrafish. This chapter encompasses basic techniques used to study bacterial infection within larval zebrafish, including embryonic zebrafish maintenance, injections of morpholino oligonucleotides, intravenous injections of bacterial suspensions, and fluorescence imaging of infected zebrafish. Specific methods for studying bacterial within-host population dynamics are also described.

Key words

Zebrafish Infection Bacterial population dynamics Fluorescence microscopy 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Krebs InstituteUniversity of SheffieldSheffieldUK
  2. 2.Department of Molecular Biology and BiotechnologyUniversity of SheffieldSheffieldUK
  3. 3.Bateson CentreUniversity of SheffieldSheffieldUK
  4. 4.Department of Infection, Immunity and Cardiovascular DiseaseUniversity of SheffieldSheffieldUK

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