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LC-MS/MS Analysis of Bile Acids

  • Sabrina Krautbauer
  • Gerhard Liebisch
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

Key words

Tandem mass spectrometry Electrospray ionization Bile acids Quantification Internal standards Method Liquid chromatography Isomer separation 

Notes

Acknowledgments

This work was supported by the “Stiftung für Pathobiochemie und Molekulare Diagnostik” and by the European Union’s FP7 Programme MyNewGut (Grant Agreement Number 613979).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Institute of Clinical Chemistry and Laboratory MedicineUniversity of RegensburgRegensburgGermany

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