LC-MS/MS Analysis of Bile Acids
Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.
Key wordsTandem mass spectrometry Electrospray ionization Bile acids Quantification Internal standards Method Liquid chromatography Isomer separation
This work was supported by the “Stiftung für Pathobiochemie und Molekulare Diagnostik” and by the European Union’s FP7 Programme MyNewGut (Grant Agreement Number 613979).
- 5.Scherer M, Gnewuch C, Schmitz G, Liebisch G (2009) Rapid quantification of bile acids and their conjugates in serum by liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 877(30):3920–3925. https://doi.org/10.1016/j.jchromb.2009.09.038CrossRefPubMedGoogle Scholar