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Shotgun Lipidomics Approach for Clinical Samples

  • Lars F. Eggers
  • Dominik SchwudkeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells.

Key words

Shotgun lipidomics High-resolution mass spectrometry Clinical samples Lipid extraction Lipid identification 

Abbreviations

CE

Cholesteryl ester

Cer

Ceramide

DAG

Diacylglycerol

FT-ICR

Fourier transform ion cyclotron resonance

HexCer

Hexosyl ceramide

LPC

Lysophosphatidylcholine

MAG

Monoacylglycerol

MFQL

Molecular fragmentation query language

MS

Mass spectrometry

MS2

Tandem mass spectrometry

MTBE

Methyl tert-butyl ether (UPAC, tert-butyl methyl ether)

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

PI

Phosphatidylinositol

PS

Phosphatidylserine

TAG

Triacylglycerol

TOF

Time of flight

Notes

Acknowledgments

The research was supported by funds of the EXC 306 Inflammation at Interfaces, German Centre for Infection Research (DZIF TTU-TB), German Centre for Lung Research, Airway Research Center North (ARCN), and Lipidomics Informatics for Life-Sciences (LIFS) of the German Network for Bioinformatics Infrastructure (de.NBI).

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Division of Bioanalytical ChemistryResearch Center BorstelBorstelGermany

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