Assessment of the Effects of a Wireless Neural Stimulation Mediated by Piezoelectric Nanoparticles
Wireless neuronal stimulation, mediated by ultrasounds and piezoelectric nanoparticles, represents an unprecedented approach aimed at cell activation. Recently, we demonstrated that barium titanate nanoparticles behave as excellent nanotransducers, by eliciting specific cell response following treatment with ultrasounds. In this chapter, we describe in detail the techniques exploited to investigate the nanoparticle/cell interactions and the activation of the neuronal-like cultures in terms of sodium and calcium fluxes.
Key wordsBarium titanate nanoparticles Ultrasounds Piezoelectricity SH-SY5Y cells Calcium imaging Sodium imaging
The authors gratefully thank Mr. Piero Narducci (Department of Chemical Engineering, University of Pisa, Pisa, Italy) for XRD technical assistance. This research was partially supported by the Italian Ministry of Health Grant Number RF-2011-02350464 (to G.C.), by the JSPS KAKENHI Grant Number 26107717 (to M.S.), and by the JSPS Core-to-Core Program, A. Advanced Research Networks (to M.S.).
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