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Peptidomics pp 395-405 | Cite as

Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss

  • Igor Fesenko
  • Regina Khazigaleeva
  • Vadim Govorun
  • Vadim Ivanov
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1719)

Abstract

Here, we report our approach to peptidomic analysis of the plant object which led to structure elucidation of the title peptides. P. patens samples were extracted under conditions preventing proteolytic digestion by endogenous proteases. The extracts were fractionated on size exclusion columns and the peptide fractions subjected to LC-MS/MS analysis. Mass spectra datasets were analyzed for the presence of peptides derived from the proteins encoded by the moss genome. Experimental details are given for each step, selected chromatograms and mass-spectra are presented in figures.

Key words

Physcomitrella patens Peptidomics Size exclusion chromatography Mass spectrometry 

Notes

Acknowledgments

This work was supported by the Russian Science Foundation (project No. 14-50-00131).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Igor Fesenko
    • 1
  • Regina Khazigaleeva
    • 1
  • Vadim Govorun
    • 1
    • 2
  • Vadim Ivanov
    • 1
  1. 1.Laboratory of Proteomics, Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Laboratory of the Proteomic AnalysisResearch Institute for Physico-Chemical MedicineMoscowRussia

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