Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Urine is a sample of choice for noninvasive biomarkers search because it is easily available in large amounts and its molecular composition provides information on processes in the organism. The high potential of urine peptidomics has been demonstrated for clinical purpose. Several mass spectrometry based approaches have been successfully applied for urine peptidome analysis and potential biomarkers search. Summarizing literature data and our own experience we developed a protocol for comprehensive urine peptidome analysis. The technology includes several stages and consists of urine sample preparation by size exclusion chromatography and identification of featured peptides by nano-HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry, semiquantitative and statistical data analysis.
Key wordsUrine peptidome Size exclusion chromatography FTICR MS LC-MS/MS
The study was supported by RFBR grants no. 16-54-21011_SNF_а, 17-08-01537 A and SNF grant no. SNF IZLRZ3_163911.
- 13.Carty DM, Siwy J, Brennand JE et al (2011) Urinary proteomics for prediction of preeclampsia. Hypertension 57:561–569. https://doi.org/10.1161/HYPERTENSIONAHA.110.164285 CrossRefPubMedGoogle Scholar
- 16.Thevenot EA, Roux A, Xu Y et al (2015) Analysis of the human adult urinary metabolome variations with age, body mass index, and gender by implementing a comprehensive workflow for univariate and OPLS statistical analyses. J Proteome Res 14:3322–3335. https://doi.org/10.1021/acs.jproteome.5b00354 CrossRefPubMedGoogle Scholar