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Proteomics Method to Identification of Protein Profiles in Exosomes

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Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

Exosomes are membrane-bound nanovesicles that transport molecular signals (e.g., proteins) between cells and are released from a wide range of cells, including the human placenta. Interestingly, the levels of exosomes present in maternal circulation are higher in preeclamptic pregnancies and their protein content profile change in response to the microenvironment milieu. Through the discovery of candidate biomarkers, mass spectrometry (MS)-based proteomics may provide a better understanding of the pathophysiology underlying pregnancy-associated disorders. With advances in sample preparation techniques, computational methodologies, and bioinformatics, MS-based proteomics have addressed the challenge of identifying and quantifying thousands of proteins and peptides from a variety of complex biological samples. Despite increasing interest in biomarker diagnostics, the complex nature of biological matrices (e.g., plasma) poses a challenge for candidate biomarker discovery. Here we describe a workflow to prepare exosomes for proteomic analysis.

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Acknowledgments

CS was in receipt of a Lions Medical Research Foundation Fellowship. This study was supported by The Lions Medical Research Foundation, UQ ECR award, Royal Brisbane and Women’s Foundation, Diabetes Australia, and UQ-Ochsner Seed Grant. The ISO17025 accredited research facility was supported by grants from Therapeutics Innovation Australia and the National Collaborative Research Infrastructure Strategy.

This review is supported partly by funding from the Lions Medical Research Foundation (LMRF), The University) of Queensland and Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT 1170809), Chile.

Author information

Authors and Affiliations

  1. Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women’s Hospital, The University of Queensland, Bldg. 71/918, Royal Brisbane Hospital, Brisbane, QLD, 4029, Australia

    Andrew Lai, Vyjayanthi Kinhal, Zarin Nuzhat, Gregory E. Rice & Carlos Salomon

  2. Division of Maternal-Fetal Medicine & Perinatal Research, Department of Obstetrics & Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, USA

    Ramkumar Menon

  3. Department of Biochemistry & Molecular Biology, The University of Texas Medical Branch at Galveston, Galveston, TX, USA

    Ramkumar Menon & Carlos Salomon

  4. Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA, USA

    Gregory E. Rice

  5. Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepción, Chile

    Carlos Salomon

Authors
  1. Andrew Lai
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  2. Vyjayanthi Kinhal
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  3. Zarin Nuzhat
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  4. Ramkumar Menon
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  5. Gregory E. Rice
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  6. Carlos Salomon
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Corresponding author

Correspondence to Carlos Salomon .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Lai, A., Kinhal, V., Nuzhat, Z., Menon, R., Rice, G.E., Salomon, C. (2018). Proteomics Method to Identification of Protein Profiles in Exosomes. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

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