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Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria

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Bacterial Multidrug Exporters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1700))

Abstract

Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based detection of efflux activity at the single cell level, and collection of single cells from droplet and subsequent gene analysis are described in detail.

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Author information

Authors and Affiliations

  1. Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan

    Ryota Iino

  2. Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), Kanagawa, 240-0193, Japan

    Ryota Iino

  3. Technical Division, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan

    Shouichi Sakakihara

  4. Department of Biomolecular Science and Regulation, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan

    Yoshimi Matsumoto

  5. Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan

    Kunihiko Nishino

  6. Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan

    Kunihiko Nishino

Authors
  1. Ryota Iino
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  2. Shouichi Sakakihara
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  3. Yoshimi Matsumoto
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  4. Kunihiko Nishino
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Corresponding author

Correspondence to Ryota Iino .

Editor information

Editors and Affiliations

  1. Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan

    Akihito Yamaguchi

  2. Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan

    Kunihiko Nishino

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Iino, R., Sakakihara, S., Matsumoto, Y., Nishino, K. (2018). Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria. In: Yamaguchi, A., Nishino, K. (eds) Bacterial Multidrug Exporters. Methods in Molecular Biology, vol 1700. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7454-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7454-2_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7452-8

  • Online ISBN: 978-1-4939-7454-2

  • eBook Packages: Springer Protocols

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