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How to Assess Drug Resistance in Cancer Stem Cells

  • Maria Laura De Angelis
  • Ruggero De Maria
  • Marta Baiocchi
Part of the Methods in Molecular Biology book series (MIMB, volume 1692)

Abstract

Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

Key words

CSC Cancer stem cell culture Drug screening Drug resistance In vitro assays Colorectal cancer 

Notes

Acknowledgments

This work was financially supported by the Italian Association for Cancer Research (AIRC) 5 per Mille 9979 grant to RDM.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Maria Laura De Angelis
    • 1
  • Ruggero De Maria
    • 2
  • Marta Baiocchi
    • 1
  1. 1.Department of Oncology and Molecular MedicineIstituto Superiore di SanitàRomeItaly
  2. 2.Department of General PathologyUniversità Cattolica del Sacro CuoreRomeItaly

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