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Polyamines pp 325-336 | Cite as

Modulation of Protein Synthesis by Polyamines in Mammalian Cells

  • Keiko KashiwagiEmail author
  • Yusuke Terui
  • Kazuei Igarashi
Part of the Methods in Molecular Biology book series (MIMB, volume 1694)

Abstract

Polyamines exist mainly as RNA–polyamine complexes in cells. Thus, we looked for proteins whose synthesis is enhanced by polyamines at the level of translation in mammalian cells. Here, we describe how synthesis of Cct2 (T-complex protein 1, β-subunit, a chaperonin assisting in the folding actin, tubulin, and several other proteins) and eEF1A (one of the elongation factors of protein synthesis) is stimulated by polyamines at the level of translation. Polyamines stimulated Cct2 synthesis through the stimulation of ribosome shunting during 5′-processive scanning of 40S ribosomal subunits from the m7G-cap to the initiation codon AUG, and eEF1A synthesis through the structural change of the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Key words

Protein synthesis Polyamine modulon 5′-UTR of mRNA Ribosome shunting Complementary sequence to 18S rRNA (CR sequence) in mRNA 

Notes

Acknowledgments

We are grateful to Dr. A.J. Michael for critical reading of the manuscript prior to submission.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Keiko Kashiwagi
    • 1
    Email author
  • Yusuke Terui
    • 1
  • Kazuei Igarashi
    • 2
    • 3
  1. 1.Faculty of PharmacyChiba Institute of ScienceChibaJapan
  2. 2.Amine Pharma Research InstituteInnovation Plaza at Chiba UniversityChibaJapan
  3. 3.Graduate School of Pharmaceutical SciencesChiba UniversityChibaJapan

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