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Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling

  • Jungeun Sarah Kwon
  • Xia Wang
  • Guang YaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1686)

Abstract

Single-cell measurements combined with mathematical modeling and computer simulations are powerful tools for understanding and exploring dynamical behaviors of gene networks and cellular functions that they control. Here, we describe experimental and computational methods to study cellular quiescence and its heterogeneity at the single-cell level.

Key words

Cell cycle Quiescence Heterogeneity Fluorescent protein reporter DNA content Click-iT EdU assay Ordinary differential equation (ODE) Deterministic simulation Stochastic simulation 

Notes

Acknowledgment

This work was supported by grants from the NSF (DMS-1463137 and DMS-1418172, to G.Y.), NIH (GM-084905, a T32 fellowship to J.S.K) and the NSF of China and Anhui Province (Grant No. 31500676 and 1508085SQC202, to X.W.).

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Molecular and Cellular BiologyUniversity of ArizonaTucsonUSA

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