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Erratum to: Ion Binding to Transport Proteins using Isothermal Titration Calorimetry

  • Shian Liu
  • Steve W. Lockless
Erratum
Part of the Methods in Molecular Biology book series (MIMB, volume 1684)
In chapter 22 titled “Ion Binding to Transport Proteins using Isothermal Titration Calorimetry” Fig. 1 was inadvertently published with a horizontal line across the figure. This has been corrected to reflect as follows:
Fig. 1

Schematic of the ITC instrument and two binding regimes. (a) The relevant portion of the ITC instrument includes two parts, a calorimetry titration syringe filled with ligand and a thermally controlled chamber filled with the ion transport protein. During the injection, the ligand is rapidly mixed with the protein and the heat exchange is recorded in thermograms (b, c) (top). Integrating the heat exchange from each injection yields binding isotherms (b, c) (bottom) that are fit to one set-of-sites functions (Eq. 1). The two isotherms are simulated with [MT] = [protein] = 100 μM, [XT] = [ligand] = 1 mM (b) or 20 mM (c), ΔH = −3 kcal/mol and n = 1 with the affinities indicated in the panels

Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of BiologyTexas A&M UniversityCollege StationUSA
  2. 2.Janelia Research CampusHoward Hughes Medical InstituteAshburnUSA

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