Ion Binding to Transport Proteins using Isothermal Titration Calorimetry

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1684)

Abstract

Isothermal titration calorimetry (ITC) is an emerging, label-free technology used to measure ligand binding to membrane proteins. This technology utilizes a titration calorimeter to measure the heat exchange upon ligands binding to proteins, the magnitude of which is based on the overall enthalpy of the reaction. In this protocol, the steps we and others use to measure ion binding to ion transport proteins are described.

Key words

Isothermal titration calorimetry Channel Transporter Ion binding Equilibrium 
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Notes

Acknowledgment

This work was supported by The Welch Foundation grant A-1742 and Texas A&M Startup Funds to S.W.L.

Supplementary material

419924_1_En_22_MOESM1_ESM.xlsx (27 kb)
Table S1 (XLSX 26 kb)

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of BiologyTexas A&M UniversityCollege StationUSA
  2. 2.Janelia Research CampusHoward Hughes Medical InstituteAshburnUSA

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