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Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes

  • Václaclav Bačovský
  • Roman Hobza
  • Boris VyskotEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1675)

Abstract

Significant advances in chromosome preparation and other techniques have greatly increased the potential of plant cytogenetics in recent years. Increase in longitudinal resolution using DNA extended fibers as well as new developments in imaging and signal amplification technologies have enhanced the ability of FISH to detect small gene targets. The combination of fluorescence in situ hybridization with immunocytochemistry allows the investigation of cell events, chromosomal rearrangements and chromatin features typical for plant nuclei. Chromosome manipulation techniques using microdissection and flow sorting have accelerated the analysis of complex plant genomes. Together, the different cytogenetic approaches are invaluable for the unravelling of detailed structures of plant chromosomes, which are of utmost importance for the study of genome properties, DNA replication and gene regulation. In this technical review, different cytogenetic approaches are discussed for the analysis of plant chromosomes, with a focus on mitotic chromosomes.

Key words

Cell synchronization Chromosome spreads Laser microdissection Cytogenetics FISH Immunostaining Antibodies 

Notes

Acknowledgments

This research was supported by the Czech Science Foundation (grant 16-08698S). We greatly thank to Dr. Alexander Oulton for English revision of this chapter. We would like to thank to Dr. Jiri Siroky and Veronika Balounova for valuable comments.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Václaclav Bačovský
    • 1
  • Roman Hobza
    • 1
  • Boris Vyskot
    • 1
    Email author
  1. 1.Department of Plant Developmental Genetics, Institute of BiophysicsCzech Academy of SciencesBrnoCzech Republic

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