Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells

  • Toshifumi AsanoEmail author
  • Toru Ishizuka
  • Hiromu Yawo
Part of the Methods in Molecular Biology book series (MIMB, volume 1668)


Optogenetic techniques are powerful tools for manipulating biological processes in identified cells using light under high temporal and spatial resolutions. Here, we describe an optogenetic training strategy to promote morphological maturation and functional development of skeletal muscle cells in vitro. Optical stimulation with a rhythmical frequency facilitates specific structural alignment of sarcomeric proteins. Optical stimulation also depolarizes the membrane potential, and induces contractile responses in synchrony with the given pattern of light pulses. These results suggest that optogenetic techniques can be employed to manipulate activity-dependent processes during myogenic development and control contraction of photosensitive skeletal muscle cells with high temporal and special precision.

Key words

Optical stimulation Channelrhodopsin Optogenetics Myogenesis Muscle contraction C2C12 



This work was supported by Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) research Fellow from JSPS and Scientific Research (KAKENHI) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan and Inochinoiro ALS Research Foundation.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Cell Biology, Graduate School of Medical and Dental SciencesTokyo Medical and Dental University (TMDU)TokyoJapan
  2. 2.Department of Developmental Biology and NeuroscienceTohoku University Graduate School of Life SciencesSendaiJapan

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