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Fluorescence Microscopy of Nanochannel-Confined DNA

  • Fredrik Westerlund
  • Fredrik Persson
  • Joachim Fritzsche
  • Jason P. Beech
  • Jonas O. Tegenfeldt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1665)

Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

Key words

DNA Nanochannels Single molecule Fluorescence 

Notes

Acknowledgements

This work was supported by the European Community’s Horizon2020 under grant agreement number 634890 entitled BeyondSeq (J.O.T., J.P.B. and F.W.), the Swedish Research Council under grant number 2015-05062 (F.W.), ERA-NET EuroNanoMed II under grant number E0748601 entitled NanoDiaBac (J.O.T., J.P.B. and F.W.). J.O.T. and J.P.B. acknowledges support from NanoLund at Lund University.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Fredrik Westerlund
    • 1
  • Fredrik Persson
    • 2
  • Joachim Fritzsche
    • 1
  • Jason P. Beech
    • 3
  • Jonas O. Tegenfeldt
    • 3
  1. 1.Chalmers University of TechnologyGothenburgSweden
  2. 2.Vanadis DiagnosticsSollentunaSweden
  3. 3.NanoLund and Department of PhysicsLund UniversityLundSweden

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