DIGE Analysis of Fish Tissues
Two-dimensional difference gel electrophoresis (2D-DIGE) appears to be especially useful in quantitative approaches, allowing the co-separation of proteins of control samples from proteins of treatment/disease samples on the same gel, eliminating gel-to-gel variability. The principle of 2D-DIGE is to label proteins prior to isoelectric focusing and use three spectrally resolvable fluorescent dyes, allowing the independent labeling of control and experimental samples. This procedure makes it possible to reduce the number of gels in an experiment, allowing the accurate and reproducible quantification of multiple samples. 2D-DIGE has been found to be an excellent methodical tool in several areas of fish research, including environmental pollution and toxicology, the mechanisms of development and disorders, reproduction, nutrition, evolution, and ecology.
Key words2D-DIGE Minimal labeling CyDye Fish Tissue
This work was supported by a Projects: 2011/01/D/NZ9/00619; 2011/01/D/NZ9/00628; 2015/17/B/NZ9/01542 and 2016/21/B/NZ9/03620 from the National Science Centre, as well as funds appropriated to the Institute of Animal Reproduction and Food Research, Polish Academy of Sciences.
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