Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
Single-molecule localization microscopy allows the ability to image fluorescence labeled molecular targets at nanoscale resolution. However, for many biological questions the ability to provide tissue and cellular context in addition to these high resolution data is eminently informative. Here, we describe a procedure to achieve this aim by correlatively imaging human cardiac tissue first at the nanoscale with direct stochastic optical reconstruction microscopy (dSTORM) and then at the diffraction limit with conventional confocal microscopy.
Key wordsSuper-resolution microscopy Confocal imaging Fluorescence Multiscale data Heart Antibodies Correlative imaging Human cardiac tissue
This work was supported by Health Research Council of New Zealand (HRC.govt.nz) grant 12/240, a Human Frontier Science Programme Award and EPSRC grant EP/N008235/1 to CS.
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