Correlative Single-Molecule Localization Microscopy and Confocal Microscopy

  • Christian SoellerEmail author
  • Yufeng Hou
  • Isuru D. Jayasinghe
  • David Baddeley
  • David Crossman
Part of the Methods in Molecular Biology book series (MIMB, volume 1663)


Single-molecule localization microscopy allows the ability to image fluorescence labeled molecular targets at nanoscale resolution. However, for many biological questions the ability to provide tissue and cellular context in addition to these high resolution data is eminently informative. Here, we describe a procedure to achieve this aim by correlatively imaging human cardiac tissue first at the nanoscale with direct stochastic optical reconstruction microscopy (dSTORM) and then at the diffraction limit with conventional confocal microscopy.

Key words

Super-resolution microscopy Confocal imaging Fluorescence Multiscale data Heart Antibodies Correlative imaging Human cardiac tissue 



This work was supported by Health Research Council of New Zealand ( grant 12/240, a Human Frontier Science Programme Award and EPSRC grant EP/N008235/1 to CS.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Christian Soeller
    • 1
    • 2
    Email author
  • Yufeng Hou
    • 2
    • 4
  • Isuru D. Jayasinghe
    • 3
  • David Baddeley
    • 5
    • 6
  • David Crossman
    • 2
  1. 1.Living Systems Institute and Biomedical PhysicsUniversity of ExeterExeterUK
  2. 2.Department of PhysiologyUniversity of AucklandAucklandNew Zealand
  3. 3.School of Biomedical SciencesUniversity of LeedsLeedsUK
  4. 4.Institute of Experimental Medical ResearchOslo University Hospital UllevålUniversity of OsloNorway
  5. 5.Auckland Bioengineering InstituteUniversity of AucklandAucklandNew Zealand
  6. 6.Department of Cell BiologyYale UniversityNew HavenUSA

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