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Polymer-Based Purification of Extracellular Vesicles

  • Peter N. Brown
  • Hang Yin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Lipid particles, including exosomes (extracellular vesicles [EVs]), are released from cells in vivo and in vitro. The contents of these EVs can be indicative of disease and therefore be utilized in diagnostic biophysical or biochemical assays. To make use of EVs in this way, methods for purification and quantification are required which vary depending on their particles origin and concentration. This chapter provides an overview of EV purification techniques and provides detailed instructions on the purification of EVs by polymer based precipitation and subsequent quantification. The subsequent quantification and characterization of these EVs also presents a challenge, as limited methods are capable of detecting EVs due to their small size.

Key words

Exosome production Extracellular vesicles Exosome purification Polymer based EV precipitation Electron microscopy Immunolabeling 

Notes

Acknowledgment

We thank the National Institutes of Health and Cancer Research UK for financial supports (NIH R01GM103843 and C596/A17096).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Drug Discovery ProgrammeBeatson Institute for Cancer ResearchGlasgowUK
  2. 2.Department of Chemistry & BiochemistryUniversity of Colorado BoulderBoulderUSA
  3. 3.BioFrontiers InstituteUniversity of Colorado BoulderBoulderUSA

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