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Characterization of Extracellular Vesicles by Flow Cytometry

  • Virginia Camacho
  • Vasilis Toxavidis
  • John C. Tigges
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Here, we describe a comprehensive methodology for the setup and standardization of EV analysis using nanoscale flow cytometry. Controls of different size ranges, fluorescent intensities, and materials can be used to set up distribution curves that are then used for instrument optimization and as a reference guide. Using these controls, flow cytometry instruments can be primed for the detection, analysis, and sorting of specific EV populations. This allows for cross platform comparison and the ability to monitor both quality control (QC) and quality assurance (QA). The method here describes the use of nanoparticles to optimize a flow cytometer for small particle detection. It also outlines the procedures necessary to recover EVs for downstream applications.

Key words

Extracellular vesicles Small particle detection Nanoscale Flow cytometry methods Nanoparticle sorting Submicron 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Virginia Camacho
    • 1
  • Vasilis Toxavidis
    • 1
  • John C. Tigges
    • 1
    • 2
  1. 1.Beth Israel Deaconess Medical Center, Harvard Medical SchoolBostonUSA
  2. 2.Flow Cytometry Core, Beth Israel Deaconess Medical CenterBostonUSA

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