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Characterization of Extracellular Vesicles by Surface Plasmon Resonance

  • Hyungsoon Im
  • Katherine Yang
  • Hakho Lee
  • Cesar M. Castro
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Surface plasmon resonance (SPR ) enables real-time, label-free detection of ligand binding to target receptors immobilized on a sensing surface. SPR has emerged as a promising technique for extracellular vesicle (EV) characterization with its label-free detection scheme, and exquisite sensitivity. Among the various system configurations, nanohole-based SPR sensors are of particular interest because of their simple optical setup, tunability, and scalability. Here, we describe the characterization of circulating EVs or exosomes from human clinical samples using a nanohole-based SPR sensor, named nPLEX (nano-plasmonic exosome) sensing.

Key words

Surface plasmon resonance Periodic nanoholes Label-free detection Protein analysis Clinical specimens 

Notes

Acknowledgment

This work was supported in part by US NIH Grants R01-HL113156 (to H.L.), K12CA087723-11 A1 (to C.M.C.) and 1K99CA201248-01 (to H.I.), the Massachusetts General Hospital Physician Scientist Development Award (to C.M.C.), and the Department of Defense Ovarian Cancer Research Program Award W81XWH-14-1-0279 (to H.L.)

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Hyungsoon Im
    • 1
  • Katherine Yang
    • 1
  • Hakho Lee
    • 1
  • Cesar M. Castro
    • 1
    • 2
  1. 1.Cancer Program, MGH Center for Systems Biology, Massachusetts General HospitalHarvard Medical SchoolBostonUSA
  2. 2.Division of Hematology-Oncology, Massachusetts General HospitalHarvard Medical SchoolBostonUSA

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