Characterization of Extracellular Vesicles by Surface Plasmon Resonance
Surface plasmon resonance (SPR ) enables real-time, label-free detection of ligand binding to target receptors immobilized on a sensing surface. SPR has emerged as a promising technique for extracellular vesicle (EV) characterization with its label-free detection scheme, and exquisite sensitivity. Among the various system configurations, nanohole-based SPR sensors are of particular interest because of their simple optical setup, tunability, and scalability. Here, we describe the characterization of circulating EVs or exosomes from human clinical samples using a nanohole-based SPR sensor, named nPLEX (nano-plasmonic exosome) sensing.
Key wordsSurface plasmon resonance Periodic nanoholes Label-free detection Protein analysis Clinical specimens
This work was supported in part by US NIH Grants R01-HL113156 (to H.L.), K12CA087723-11 A1 (to C.M.C.) and 1K99CA201248-01 (to H.I.), the Massachusetts General Hospital Physician Scientist Development Award (to C.M.C.), and the Department of Defense Ovarian Cancer Research Program Award W81XWH-14-1-0279 (to H.L.)
- 13.Wittenberg NJ, Im H, Xu X, Wootla B, Watzlawik J, Warrington AE, Rodriguez M, Oh S-H (2012) High-affinity binding of remyelinating natural autoantibodies to myelin-mimicking lipid bilayers revealed by nanohole surface plasmon resonance. Anal Chem 84:6031–6039CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Thery C, Amigorena S, Raposo G, Clayton A (2006) Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol Chapter 3:Unit 3.22Google Scholar