MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

Part of the Methods in Molecular Biology book series (MIMB, volume 1659)


MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.

Key words

MutRenSeq Gene cloning NB-LRR Resistance gene Target enrichment sequencing 



The authors thank the support by the Gatsby Charitable Foundation, UK, the Two Blades Foundation, USA and the Biotechnology and Biological Sciences Research Council, UK, Grant numbers BB/H019820/1, BB/L009293/1 and BB/L011794/1.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.John Innes Centre, Norwich Research ParkNorwichUK
  2. 2.The Sainsbury Laboratory, Norwich Research ParkNorwichUK

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