MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.
Key wordsMutRenSeq Gene cloning NB-LRR Resistance gene Target enrichment sequencing
The authors thank the support by the Gatsby Charitable Foundation, UK, the Two Blades Foundation, USA and the Biotechnology and Biological Sciences Research Council, UK, Grant numbers BB/H019820/1, BB/L009293/1 and BB/L011794/1.