MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

  • Burkhard Steuernagel
  • Kamil Witek
  • Jonathan D. G. Jones
  • Brande B. H. Wulff
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1659)

Abstract

MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.

Key words

MutRenSeq Gene cloning NB-LRR Resistance gene Target enrichment sequencing 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Burkhard Steuernagel
    • 1
  • Kamil Witek
    • 2
  • Jonathan D. G. Jones
    • 2
  • Brande B. H. Wulff
    • 1
  1. 1.John Innes Centre, Norwich Research ParkNorwichUK
  2. 2.The Sainsbury Laboratory, Norwich Research ParkNorwichUK

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