RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity

  • Renee C. Ireton
  • Courtney Wilkins
  • Michael GaleJr.
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1656)

Abstract

Pathogen recognition receptors (PRR)s and their cognate pathogen-associated molecular pattern (PAMP) represent the basis of innate immune activation and immune response induction driven by the host-pathogen interaction that occurs during microbial infection in humans and other animals. For RNA virus infection such as hepatitis C virus (HCV) and others, specific motifs within viral RNA mark it as nonself and visible to the host as a PAMP through interaction with RIG-I-like receptors including retinoic inducible gene-I (RIG-I). Here, we present methods for producing and using HCV PAMP RNA as a molecular tool to study RIG-I and its signaling pathway, both in vitro and in vivo, in innate immune regulation.

Key words

RNA PAMP Innate immunity Rig-I Pathogen recognition receptor 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Renee C. Ireton
    • 1
  • Courtney Wilkins
    • 1
  • Michael GaleJr.
    • 1
  1. 1.Department of Immunology, Center for Innate Immunity and Immune DiseaseUniversity of Washington School of MedicineSeattleUSA

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