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Protein Arrays I: Antibody Arrays

  • Yulin Yuan
  • Zuan-Tao Lin
  • Hongting Wang
  • Xia Hong
  • Mikala Heon
  • Tianfu Wu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1654)

Abstract

Antibody arrays represent one of the very early protein array systems where antibodies are used to capture and detect target proteins in a high-throughput platform. The development of high-quality antibodies, nanomaterial-based novel detection probes, as well as innovative imaging technologies and computational tools has tremendously improved the sensitivity, specificity, and robustness of antibody arrays during the past decade. In this protocol we will incorporate the most updated innovations and developments of antibody arrays into the step-by-step experimental procedures. This includes antibody printing, sample preparation, array detection, as well as imaging and data analysis. Antibody array could be used for cytokine profiling or mapping of phosphorylation, glycosylation, or other post-translational modifications of target proteins.

Key words

Antibody array Cytokine profiling Kinome mapping Glycome mapping Biomarkers 

Notes

Acknowledgments

This work was partly supported by a grant from the Lupus Research Institute to T.W. and a startup fund from the University of Houston to T.W.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Yulin Yuan
    • 1
    • 2
  • Zuan-Tao Lin
    • 1
  • Hongting Wang
    • 1
    • 3
  • Xia Hong
    • 1
    • 4
  • Mikala Heon
    • 1
  • Tianfu Wu
    • 1
  1. 1.Department of Biomedical EngineeringUniversity of HoustonHoustonUSA
  2. 2.Department of Clinical LaboratoryThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanningChina
  3. 3.National pharmacology Laboratory of Chinese MedicineCollege of Basic Medical Sciences, Wannan Medical CollegeWuhuChina
  4. 4.Department of NursingFujian Health CollegeFuzhouChina

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