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Metabolic Engineering of Photorespiration

  • Martin K. M. Engqvist
  • Veronica G. MaurinoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1653)

Abstract

The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.

Key words

PCR amplification Binary vectors Arabidopsis transformation Agrobacterium tumefaciens 

Notes

Acknowledgements

This work was supported by grants of the Deutsche Forschungsgemeinschaft, MA2379/4-1, FOR 1186, and EXC 1028 to V.G.M.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Biology and Biological Engineering, Division of Systems and Synthetic BiologyChalmers University of TechnologyGöteborgSweden
  2. 2.Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Cluster of Excellence on Plant Sciences (CEPLAS)Heinrich-Heine-UniversitätDüsseldorfGermany

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