Chicken Induced Pluripotent Stem Cells: Establishment and Characterization
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In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
Key wordsChicken Induced pluripotent stem cells Transfection
This work was supported by grants from INRA and from ANR with the CRB-ANIM project (CRB-ANIM-ANR-11-INBS-0003).
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