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RNA Detection pp 305-318 | Cite as

Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe

  • Shin-ichi SatoEmail author
  • Kenji Yatsuzuka
  • Yousuke Katsuda
  • Motonari UesugiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1649)

Abstract

Live-cell imaging of mRNA dynamics is increasingly important to understanding spatially restricted gene expression. We recently developed a convenient and versatile method that uses a gene-specific RNA aptamer and a fluorescent probe to enable spatiotemporal imaging of endogenous mRNAs in living cells. The method was validated by live-cell imaging of the endogenous mRNA of β-actin. The new RNA-imaging technology might be useful for live-cell imaging of any RNA molecules.

Key words

Live-cell imaging mRNA RNA aptamer Small molecule Chemical biology 

Notes

Acknowledgment

This work was supported in part by JSPS (26220206 to M.U. and 26440005 to S.S.), ZE Research Program, IAE (ZE27B-18 to S.S.), and an iCeMS research acceleration grant. iCeMS is supported by World Premier International Research Center Initiative (WPI), MEXT, Japan. This work was inspired by the international and interdisciplinary environments of the JSPS Core-to-Core Program, “Asian Chemical Biology Initiative.” We also thank M. Nakashima for manuscript preparation.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Institute for Integrated Cell-Material Sciences (WPI-iCeMS)Kyoto UniversityUji, KyotoJapan
  2. 2.Institute for Chemical ResearchKyoto UniversityUji, KyotoJapan

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