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Determination of Fibrinogen and Thrombin Time (TT)

  • Anetta UndasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1646)

Abstract

Fibrinogen is measured in plasma most commonly using the Clauss method, based on the comparison of thrombin clotting times of dilutions of plasma against a plasma standard. Thrombin time (TT) is a coagulation assay, which reflects the conversion of fibrinogen to fibrin after addition of thrombin reagent. Measurement of clottable fibrinogen and TT allows detecting inborn (congenital) and acquired qualitative and quantitative disorders of fibrinogen that can lead to thrombotic or bleeding events.

Key words

Clotting Fibrinogen Fibrin Plasma Thrombin time 

References

  1. 1.
    Undas A (2014) Fibrin clot properties and their modulation in thrombotic disorders. Thromb Haemost 112:32–42CrossRefPubMedGoogle Scholar
  2. 2.
    Mosesson MW (2005) Fibrinogen and fibrin structure and functions. J Thromb Haemost 3:1894–1904CrossRefPubMedGoogle Scholar
  3. 3.
    Mackie IJ, Kitchen S, Machin SJ, Lowe GD, Haemostasis and Thrombosis Task Force of the British Committee for Standards in Haematology (2003) Guidelines on fibrinogen assays. Br J Haematol 121:396–404CrossRefPubMedGoogle Scholar
  4. 4.
    Multifibren U (2008) Package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg Germany. Edition May 2008Google Scholar
  5. 5.
    Clauss A (1957) Gerinnungsphysiologische Schnellmethode zu Bestimmung des Fibrinogens. Acta Haematol 17:237–245CrossRefPubMedGoogle Scholar
  6. 6.
    Undas A (2016) How to assess fibrinogen levels and fibrin clot properties in clinical practice? Semin Thromb Hemost 42:381–388CrossRefPubMedGoogle Scholar
  7. 7.
    Adcock DM, Bethel MA, Macy PA (2002) Coagulation handbook. Esoterix, Inc. Laboratory Services. pp 134–136Google Scholar
  8. 8.
    Chandler WL (2009) Initial evaluation of hemostasis: reagent and method selection. In: Kitchen S, Olson JD, Preston FE (eds) Quality in laboratory hemostasis and thrombosis. Wiley-Blackwell, Oxford, pp 63–71CrossRefGoogle Scholar
  9. 9.
    Fickenscher K (1998) Thrombin time (TT) test. In: Lothar T (ed) Clinical laboratory diagnostics: use and assessment of clinical laboratory results. TH-Books Verlagsgesellschaft mbH, Frankfurt/Main, Germany, pp 604–605Google Scholar
  10. 10.
    Flanders MM, Crist R, Rodgers GM (2003) Comparison of five thrombin time reagents. Clin Chem 49:169–712CrossRefPubMedGoogle Scholar
  11. 11.
    Ignjatovic V (2013) Thrombin clotting time. Methods Mol Biol 992:131–138CrossRefPubMedGoogle Scholar
  12. 12.
    Siemens Healthcare Diagnostics Products GmbH (2009) Fibrinogen Calibrator Kit package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition October 2009Google Scholar
  13. 13.
    Siemens Healthcare Diagnostics Products GmbH (2015) Control Plasma N package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition July 2015Google Scholar
  14. 14.
    Siemens Healthcare Diagnostics Products GmbH (2007) Control Plasma P package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition November 2007Google Scholar
  15. 15.
    Siemens Healthcare Diagnostics Products GmbH (2010) BC Thrombin Reagent package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition March 2010Google Scholar
  16. 16.
    Monagle P, Barnes C, Ignjatovic V, Furmedge J, Newall F, Chan A, De Rosa L, Hamilton S, Ragg P, Robinson S, Auldist A, Crock C, Roy N, Rowlands S (2006) Developmental haemostasis. Impact for clinical haemostasis laboratories. Thromb Haemost 95:362–372PubMedGoogle Scholar
  17. 17.
    Cunningham MT, Brandt JT, Laposata M, Olson JD (2002) Laboratory diagnosis of dysfibrinogenemia. Arch Pathol Lab Med 126:499–505PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Institute of CardiologyJagiellonian University Medical CollegeCracowPoland

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