Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood
Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.
Key wordsPlatelet function Flow cytometry Whole blood Glycoprotein receptor deficiency Dense granule release Procoagulant platelets Calcium flux Platelet-leukocyte aggregates
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