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Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

  • Leonardo PasalicEmail author
  • Gabrielle J. Pennings
  • David Connor
  • Heather Campbell
  • Leonard Kritharides
  • Vivien M. Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1646)

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

Key words

Platelet function Flow cytometry Whole blood Glycoprotein receptor deficiency Dense granule release Procoagulant platelets Calcium flux Platelet-leukocyte aggregates 

References

  1. 1.
    Bennett JS (2001) Platelet-fibrinogen interactions. Ann N Y Acad Sci 936(1):340–354. doi: 10.1111/j.1749-6632.2001.tb03521.x CrossRefPubMedGoogle Scholar
  2. 2.
    Sangkuhl K, Shuldiner AR, Klein TE, Altman RB (2011) Platelet aggregation pathway. Pharmacogenet Genomics 21(8):516–521. doi: 10.1097/FPC.0b013e3283406323 CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Ferroni P, Vazzana N, Riondino S, Cuccurullo C, Guadagni F, Davi G (2012) Platelet function in health and disease: from molecular mechanisms, redox considerations to novel therapeutic opportunities. Antioxid Redox Signal 17(10):1447–1485. doi: 10.1089/ars.2011.4324 CrossRefPubMedGoogle Scholar
  4. 4.
    Pearse AGE (1980) Preparative and optical technology. In: Histochemistry, theoretical and applied, vol 1, 4th edn. Churchill Livingstone, EdinburghGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Leonardo Pasalic
    • 1
    • 2
    Email author
  • Gabrielle J. Pennings
    • 3
  • David Connor
    • 4
  • Heather Campbell
    • 5
  • Leonard Kritharides
    • 3
    • 6
  • Vivien M. Chen
    • 3
    • 5
    • 7
  1. 1.Departments of Clinical and Laboratory HaematologyInstitute of Clinical Pathology and Medical Research, WestmeadHospitalWestmeadAustralia
  2. 2.Sydney Centres for Thrombosis and HaemostasisWestmeadAustralia
  3. 3.ANZAC Research InstituteUniversity of SydneySydneyAustralia
  4. 4.Blood, Stem Cell and Cancer Research UnitSt Vincent’s Centre for Applied Medical ResearchSydneyAustralia
  5. 5.Prince of Wales Clinical School, Faculty of MedicineUniversity Of New South WalesSydneyAustralia
  6. 6.Department of Cardiology, Concord Repatriation General HospitalSydneyAustralia
  7. 7.Department of HaematologyConcord Repatriation General HospitalSydneyAustralia

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